Fièvre Typhoïde | Santé Diététique
la fièvre typhoïde
S. typhi Vi
Suspension
S.
typhi Vi
Standard Serum
S. typhi Vi
Suspension for the quantitative detection of specific Salmonella antibodies in
sera S. typhi Vi
Standard Serum for the standardisation of the agglutination tests using Vi
suspensions
INTENDED USE
Remel
S. typhi Vi
Suspension (SS20/30953901) is intended for the quantitative detection of
specific Salmonella antibodies in sera for epidemiological purposes to assist
in the diagnosis of carriers.
Remel
S. typhi Vi
Standard Serum (AM30/30951401) is a freeze-dried hyperimmune horse serum
intended for the standardisation of agglutination tests using Vi suspension.
REAGENTS
DESCRIPTION, PREPARATION FOR USE AND
RECOMMENDED STORAGE CONDITIONS
Remel
S. typhi Vi
Suspension is standardised to contain
approximately 1010 bacteria per ml and is
preserved with 0.25% formalin and 0.01%thiomersal. Each bottle contains 5 ml liquid and is ready to use.
The
Remel S.
typhi Vi Standard Serum is horse serum
containing antibodies to S.
typhi Vi preserved with 0.01% thiomersal.
Stored
in the dark at 2 to 8°C the suspension and freeze-dried Standard Serum will
retain their reactivity at least until the date shown on the bottle label.
The
freeze-dried Standard Serum should be reconstituted for use in 1 ml of
distilled water and
further diluted with 3 ml saline.
Suitable
aliquots of this 1/4 dilution may be stored at –15°C to –25°C until required.
ANALYTICAL PRECAUTIONS
1. Do not use the reagents beyond the stated expiry date.
Microbiological and serological contamination must be avoided as this may cause erroneous results and
reduce product life.
2. Do not modify the test procedure, incubation times or temperatures.
3. Allow all reagents and samples to come to room temperature
(18 to 30°C) before use. After use return to 2 to 8°C in the dark.
4. Do not expose reagents to strong light during storage and incubation times.
5. Care must be taken not to cross-contaminate reagents.
6. If the suspension becomes rough or fails to agglutinate with its
homologous specific serum it should be discarded.
7. The suspension must be shaken thoroughly before use to ensure
that organisms are evenly suspended.
SPECIMEN COLLECTION, TRANSPORT AND STORAGE
SPECIMEN COLLECTION
Serum
samples may be used.
Blood
collected by venous puncture should be allowed to clot naturally.
Care
should be taken to ensure that the serum samples
are fully clotted. Do not inactivate serum
samples.
SPECIMEN TRANSPORT AND STORAGE
Store
samples at 2 to 8°C.
TEST PROCEDURE
1.
Set up a row of eight tubes for each serum tested,
including the Standard Serum.
nPipette
1.8 ml of saline into the first tube in each row and 1.0 ml into every other
tube.
nAdd
0.2 ml of test serum or diluted Standard Serum to the first tube of the
appropriate row.
nMix
the contents of tube 1 and transfer 1 ml to tube 2.
nRepeat
for each tube up to but not including tube 8, finally discarding 1 ml from tube
7.
INTENDED USE
Remel
S. typhi Vi
Suspension (SS20/30953901) is intended for the quantitative detection of
specific Salmonella antibodies in sera for epidemiological purposes to assist
in the diagnosis of carriers.
Remel
S. typhi Vi
Standard Serum (AM30/30951401) is a freeze-dried hyperimmune horse serum
intended for the standardisation of agglutination tests using Vi suspension.
REAGENTS
DESCRIPTION, PREPARATION FOR USE AND
RECOMMENDED STORAGE CONDITIONS
Remel
S. typhi Vi
Suspension is standardised to contain
approximately 1010 bacteria per ml and is
preserved with 0.25% formalin and 0.01%thiomersal. Each bottle contains 5 ml liquid and is ready to use.
The
Remel S.
typhi Vi Standard Serum is horse serum
containing antibodies to S.
typhi Vi preserved with 0.01% thiomersal.
Stored
in the dark at 2 to 8°C the suspension and freeze-dried Standard Serum will
retain their reactivity at least until the date shown on the bottle label.
The
freeze-dried Standard Serum should be reconstituted for use in 1 ml of
distilled water and
further diluted with 3 ml saline.
Suitable
aliquots of this 1/4 dilution may be stored at –15°C to –25°C until required.
ANALYTICAL PRECAUTIONS
1. Do not use the reagents beyond the stated expiry date.
Microbiological and serological contamination must be avoided as this may cause erroneous results and1. Do not use the reagents beyond the stated expiry date.
reduce product life.
2. Do not modify the test procedure, incubation times or temperatures.
3. Allow all reagents and samples to come to room temperature
(18 to 30°C) before use. After use return to 2 to 8°C in the dark.
4. Do not expose reagents to strong light during storage and incubation times.
5. Care must be taken not to cross-contaminate reagents.
6. If the suspension becomes rough or fails to agglutinate with its
homologous specific serum it should be discarded.
7. The suspension must be shaken thoroughly before use to ensure
that organisms are evenly suspended.
SPECIMEN COLLECTION, TRANSPORT AND STORAGE
SPECIMEN COLLECTION
Serum samples may be used.
Blood collected by venous puncture should be allowed to clot naturally.
Care should be taken to ensure that the serum samples
are fully clotted. Do not inactivate serum samples.
SPECIMEN COLLECTION
Serum samples may be used.
Blood collected by venous puncture should be allowed to clot naturally.
Care should be taken to ensure that the serum samples
are fully clotted. Do not inactivate serum samples.
SPECIMEN TRANSPORT AND STORAGE
Store samples at 2 to 8°C.
Store samples at 2 to 8°C.
TEST PROCEDURE
1. Set up a row of eight tubes for each serum tested,
including the Standard Serum.
nPipette 1.8 ml of saline into the first tube in each row and 1.0 ml into every other tube.
nAdd 0.2 ml of test serum or diluted Standard Serum to the first tube of the appropriate row.
1. Set up a row of eight tubes for each serum tested,
including the Standard Serum.
nPipette 1.8 ml of saline into the first tube in each row and 1.0 ml into every other tube.
nAdd 0.2 ml of test serum or diluted Standard Serum to the first tube of the appropriate row.
nMix
the contents of tube 1 and transfer 1 ml to tube 2.
nRepeat for each tube up to but not including tube 8, finally discarding 1 ml from tube 7.
nRepeat for each tube up to but not including tube 8, finally discarding 1 ml from tube 7.
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